Anionic charge sites in the kidney: Comparison of two diabetic rat models

The distribution and appearance of anionic charge sides (ACS) in the glomer ular basement membrane (GBM) and mesangium were studied in two different an imal models of diabetes mellitus (DM), the obese Zucker rat as an animal mo del of type 2 diabetes mellitus (DM) and streptozocin-induced Sprague-Dawle y rat as tin animal model of type 1 DM. Four obese Zucker rats (ZR) and four Sprague-Dawley rats were analyzed for the following parameters: number of ACS per length of lamina rare externa ( LRE), (ACS/LRE); number of ACE per length of lamina rara interna (LRI) (ACS /LRI); percent of mesangial matrix as ACS (%MMACS); percent of LRE as ACS ( %LREACS); percent of LRI as ACS (%LRIACS); length of ACS in LRI (LACSLRI); length of ACS in LRE (LACSLRE); width of ACS in LRI (WACSLRI); width of ACS in the LRE (WACSLRE); area of ACS in the LRI (AACSLRI); and the area of AC S in the LRE (AACSLRE). Statistical analyses include a one-way analysis of variance (ANOVA), Pearso n's correlation coefficient, and stepwise multiple regression. This study confirms that a loss of ACS occurs as proteinuria develops in a variety of animal models. The majority of the ACS were more prominently loc alized, and thus lost form the LRE of the GBM in DN. This study also demons trated that defined alterations in the glomerular ACS can be identified ear ly in the evolution of DN in both animal models, and that the similarity of the changes in the ACS suggest that a common pathophysiologic mechanism in duced the changes in both animal models.