Organometallic complexes with biological molecules: XIII. Organotin(IV)[meso-tetra (4-carboxyphenyl)porphinate]s and the cell cycle: A flow-cytometric approach

The cytotoxic derivatives diorganotin(IV) and triorganotin(IV) [meso-tetra( 4-carboxyphenyl)-porphinates, with stoichiometries [R2Sn](2)TPPC and [R3Sn] (4)TPPC [R=Me, Bu, Ph; TIPPC4- meso -tetra(4-carboxyphenyl)porphinate(4-)], namely bis[dimethyltin(IV)], bis[dibutyltin(IV)], bis[diphenyltin(IV)], te tra[trimethyltin(IV)],tetra[tributyltin(IV)] and tetra[triphenyltin(IV)] [m eso-tetra(4-carboxyphenyl)porphinate]s have been used to investigate their effects oil the cultured human kidney cell cycle in order to understand fur ther the origin of cell-growth inhibition induced by the above-mentioned ch emicals. The cell-cycle-dependent DNA content distribution of cultured cell s exposed to these compounds has been analyzed through how cytometry, a pot ent technique capable of probing several aspects of drug-induced cytotoxici ty. Cultured human kidney cells have been used as a model system, on the pr emise of greater physiological similarity to the human situation in vivo. C opyright (C) 1999 John Wiley & Sons, Ltd.