Optical absorption and fluorescence spectral imaging using fiber bundle image compression

Optical absorption and fluorescence spectral imaging is performed by Using the recently developed fiber-bundle image compression (FIC) technique in wh ich an entire spectral image is collected in a single scan of a charged-cou pled device (CCD) detector. Absorption imaging is demonstrated by mapping t he optical absorbance of a stained microscopic lily plant stem section. Flu orescence imaging is demonstrated by mapping shifts in the ruby R-1 fluores cence line to determine the pressure distribution in a microcrystalline rub y powder squeezed between two diamond surfaces. The advantages and limitati ons of the FIC method, relative to tunable filter and line imaging techniqu es, are discussed.