Experimental assessment of the pathogenicity of the Newcastle disease viruses from outbreaks in Great Britain in 1997 for chickens and turkeys, and the protection afforded by vaccination

The Newcastle disease virus isolated from healthy turkeys in outbreak GB 97 /6 was used to challenge 4-week-old turkeys and chickens, which were either not vaccinated or had received a single dose of Hitchner B1 live vaccine 1 4 days earlier, by one of the intramuscular, intranasal or contact routes. Similar experiments were done in 38-day-old turkeys and chickens using viru s isolated from severely sick chickens in outbreak GB 97/1, All vaccinated chickens showed low but measurable immune responses 14 days after vaccinati on, but only three of the turkeys had detectable antibodies. No vaccinated turkey or chicken showed any clinical sign after challenge with either viru s, The virus from healthy turkeys in outbreak GB 97/6 induced clinical sign s in 12/30 unvaccinated turkeys after challenge and 7/30 died. In unvaccina ted chickens, challenge with this virus produced clinical signs in 25/30 bi rds and 21/30 died. In challenge experiments with the virus from outbreak G B 97/1 in chickens, 3/30 unvaccinated turkeys showed clinical signs and all three subsequently died. In contrast, 30/30 unvaccinated chickens challeng ed with this virus showed clinical signs and died. Vaccination did not prev ent infection and excretion of either challenge virus. However, when compar ed with unvaccinated birds, vaccination reduced significantly the length of time virus was excreted and the overall proportion of swabs that were posi tive.