Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus

The kdsA gene from the hyperthermophilic bacterium Aquifex aeolicus was clo ned into a vector for expression in Escherichia coli and the kdsA gene prod uct, 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (KdsA), was over expressed under optimized growth conditions. The thermophilic KdsA was puri fied using an efficient purification procedure including a heat-treatment s tep. Purified KdsA was shown to catalyze the formation of 3-deoxy-D-manno-o ctulosonic acid 8-phosphate from phosphoenolpyruvate (PEP) and D-arabinose 5-phosphate (A 5-P) as determined from H-1 NMR analysis of the product. Ana lytical gel filtration analysis indicated the native enzyme to be oligomeri c. KdsA was extremely thermostable, exhibiting maximal activity at 95 degre es C and with half-lives of 1.5 h (90 degrees C), 8.1 h (80 degrees C), and 30.3 h (70 degrees C). KdsA appeared to follow Michaelis-Menton kinetics w ith K-m(A5-P) = 8 - 74 mu M, K-m(PEP) = 43-28 mu M, and k(cat) = 0.4-2.0 s( -1) between 60 and 90 degrees C. (C) 1999 Academic Press.