Sequence analysis of the spacer region between the RHD and RHCE genes

Citation
H. Okuda et al., Sequence analysis of the spacer region between the RHD and RHCE genes, BIOC BIOP R, 263(2), 1999, pp. 378-383
Citations number
54
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
263
Issue
2
Year of publication
1999
Pages
378 - 383
Database
ISI
SICI code
0006-291X(19990924)263:2<378:SAOTSR>2.0.ZU;2-B
Abstract
Numerous variants of the Rh blood group system, discovered by Levine and St etson in 1939, have been detected and more than forty antigens have been id entified. By performing the molecular genetic analysis of the introns as we ll as the exons in both RH genes, it was elucidated that Rh variants were g enerated by gene conversion or recombination, deletions, or mutations. For understanding the generation of many Rh variants and Rh antigens in detail, it is necessary to analyze not only the RHCE and RHD genes but also the st ructure and the physical distance between both these RH genes. In order to achieve the aforesaid purpose, the spacer region between the RHD and RHCE g enes were amplified by the long PCR method. Therefore the full spacer regio n was determined to be 12159 bp in length and contained the Alu consensus s equences and the putative CpG island. It was probable that the duplication of both RH genes occurred within about 12 kb region. Analysis of the spacer region provides new information for the research on the transcription-cont rol region, the molecular evolution of RH genes, Rh variants, and the delet ion of the RHD gene in Rh blood group system. (C) 1999 Academic Press.