Effect of resin characteristics on fluidized bed adsorption of proteins

Citation
Pr. Wright et al., Effect of resin characteristics on fluidized bed adsorption of proteins, BIOTECH PR, 15(5), 1999, pp. 932-940
Citations number
31
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
87567938 → ACNP
Volume
15
Issue
5
Year of publication
1999
Pages
932 - 940
Database
ISI
SICI code
8756-7938(199909/10)15:5<932:EORCOF>2.0.ZU;2-G
Abstract
Despite numerous advantages, fluidized bed adsorption is limited in commerc ial applications due to a poor understanding of the relationships between m ass transfer, hydrodynamics, and adsorption. To obtain a better understandi ng of these parameters, we used two commercially available resins to compar e the adsorption of lysozyme as a function of bed expansion and bulk-phase viscosity using frontal analysis. Under static conditions adsorption was we ll represented by a Langmuir isotherm. Dynamic capacities at breakthrough w ere measured and normalized relative to the equilibrium capacity of each re sin to facilitate direct comparison of resin performance under each conditi on. To quantify the impact of resin characteristics in expanded bed adsorpt ion, we carried out a comparison of mass-transfer effects using a macroporo us resin, Streamline SP, and a hyper-diffusive resin, S-HyperD LS. Both res ins are designed for fluidized bed adsorption of proteins. In this study th e results of frontal analysis showed that breakthrough was due to mass-tran sfer limitations of the adsorbing particles at expansions of 2 times the se ttled bed height. At expansions of 3 and 4 times the settled bed height, ax ial dispersion increased significantly; however mass-transfer limitations w ere still the dominant mechanistic feature contributing to early breakthrou gh at reduced dynamic capacity. Adsorption capacity under all conditions in this study was poorer for the macroporous resin than for the hyper-diffusi ve resin due to intraparticle mass-transfer limitations.