Simple and rapid screening method for microbial D-stereospecific peptidaseand esterase

Citation
Sj. Kwon et al., Simple and rapid screening method for microbial D-stereospecific peptidaseand esterase, BIOTECH TEC, 13(10), 1999, pp. 653-655
Citations number
6
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
10
Year of publication
1999
Pages
653 - 655
Database
ISI
SICI code
0951-208X(199910)13:10<653:SARSMF>2.0.ZU;2-E
Abstract
The use of a turbid plate containing Z-D-Ala-D-AlaOBzl was a convenient met hod for the screening of microorganisms with D-stereospecific peptidase act ivity as well as D-stereospecific esterase activity. This paper discusses t he suitability of the developed method and its application to molecular clo ning of D-stereospecifc peptidase and esterase genes.