A novel technique for the study of bacterial cell mechanical properties

A micromanipulation method is described for measuring the bursting forces o f bacteria and relating them to cell size. At a compression speed of 6.2 mu m s(-1), bursting forces of three samples of rapidly growing Staphylococcu s epidermis from a batch culture varied from 3 to 34 mu N with an average v alue of 13.8 mu N (standard error 0.8 mu N). Escherichia coli grown in cont inuous culture at a specific growth rate of 0.5 h(-1) had bursting forces v arying from 1 to 9 mu N with an average value of 3.6 mu N (standard error 0 .4 mu N). In squeeze-hold experiments, force relaxation was observed, which was attributed to water loss from the cells, or viscoelasticity, or both. At high compression speed, such as 6.2 mu m s(-1), this relaxation could be neglected. Micromanipulation strength measurements might be used in studie s of cell mechanical disruption and of the dependence of cell strength on c ell physiology.