Penetration of topical, oral, and combined administered ofloxacin into thesubretinal fluid

Aims-To assess the subretinal fluid (SRF) levels of ofloxacin following top ical, oral or combined administration. Methods-31 patients undergoing conventional retinal reattachment surgery we re randomly assigned to three groups. Nine patients received topical ofloxa cin, 11 patients received oral ofloxacin, and the other 11 patients receive d combined administration. Collected SRF samples were analysed for drug lev el by using high performance liquid chromatography. Results-SRF drug levels after oral and combined administration were signifi cantly higher than that after topical administration (p=0.0002 and p=0.0002 , respectively) while there was no significant difference between oral and combined administration (p=0.0844). Conclusions-Ocular bioavailability of ofloxacin in SRF after oral and combi ned administration is equivalent. The addition of oral ofloxacin to topical therapy increased drug SRF penetration sixfold.