Fluorescence in situ hybridization analysis of complex translocations in two newly diagnosed Philadelphia chromosome-positive chronic myelogenous leukemia patients
E. Rajcan-separovic et al., Fluorescence in situ hybridization analysis of complex translocations in two newly diagnosed Philadelphia chromosome-positive chronic myelogenous leukemia patients, CANC GENET, 114(1), 1999, pp. 71-74
Two different complex translocations from newly diagnosed cases of Philadel
phia chromosome-positive chronic myelogenous leukemia (CML) were characteri
zed by G-banding and fluorescence in situ hybridization (FISH) analysis. In
one case, a unique balanced t(9;22;9;11) (q34;q11;p22;q23) was identified
by G-banding, and confirmed by FISH using MBCR/ABL and painting probes. In
the second case, an apparently balanced t(19;22) was identified by G-bandin
g analysis. FISH using MBCR/ABL probe defected the fusion gene on the deriv
ative chromosome 22, indicating the involvement of chromosome 9. Further FI
SH analysis with selected painting probes showed that the t(19;22) was a re
sult of a complex translocation involving chromosomes 9, 19, 21, and 22. (C
) Elsevier Science Inc., 1999. All rights reserved.