HSP27 and MKBP translocate from the cytosolic to myofibril fraction in isch
emic rat heart as demonstrated by immunoblotting. Immunohistochemistry anal
ysis showed that ischemia enhances the Z line labeling of HSP27 and MKBP. T
wo dimensional gel electrophoresis showed that ischemia increases the hyper
phosphorylated form of HSP27. These data suggest that HSP27 and MKBP may be
involved in the Z line protection against postischemic reperfusion injury.