Cloning and characterization of vernalization-related gene (ver203F) cDNA 3 ' end

Based on the cDNA fragment sequence of vernalization-related gene verc203 c loned by differential screening in our lab, the 5' primer has been designed . The cDNA 3' end of ver203 gene (1 197 bp) has been cloned by the RACE met hod. And it is identified by Northern blotting that its expression is speci al in vernalization treatment. After comparing the sequence in the nucleoti de sequence databases of Genbank, EMBL and DDBJ, the gene has homology with Hordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate.