Gene mapping of a new coat mutation in mouse

Gene mapping of a mouse coat mutation has been investigated. First, 100 10- bp random primers were used to amplify DNA, but the mutation could not be l ocated by this method because there were no correlation between the amplifi ed products and coat phenotypes. Second, by using Idh1, Car2, Mup1, Pgb1, H bb, Es10, Es1, Mod1, Gdc1, Ce2, Es3 as genetic markers, linkage test crosse s (two-point test) consisting of intercrossing uncovered BALB/c mice (homoz ygotes) to CBA/N and C57BL/6 mice with normal hair and backcrossing the het erozygotes of the F1 to the uncovered BALB/c mice were made. It was soon ev ident that the mutation was linked to Es3 on chromosome 11. Furthermore, th ree-point test was made by using Es3 and D11Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked to Es3 with the percentage recombination of (7.89 +/- 2.19)%, and linked to D11Mit8 wi th the percentage recombination of (26.38 +/- 3.57)%. The percentage recomb ination between Es3 and D11Mit8 was (32.90 +/- 3.81)%. The mutation was nam ed Uncovered, with the symbol Uncv. According to the recombinations, the lo ci order was D11Mit8-26.30 +/- 3.57- Uncv-7.89 +/- 2.19-Es3. From the locat ion on the chromosome, it was concluded that the mutation was a new mutatio n which affected the skin and hair structure of mouse. The Uncv has entered MGD (Mouse Genome Database).