Arterial relaxation mediated by endothelium-derived hyperpolarizing factorin hypertension induced by chronic inhibition of nitric oxide synthesis

Citation
K. Kimura et al., Arterial relaxation mediated by endothelium-derived hyperpolarizing factorin hypertension induced by chronic inhibition of nitric oxide synthesis, CLIN EXP HY, 21(7), 1999, pp. 1203-1221
Citations number
40
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
CLINICAL AND EXPERIMENTAL HYPERTENSION
ISSN journal
10641963 → ACNP
Volume
21
Issue
7
Year of publication
1999
Pages
1203 - 1221
Database
ISI
SICI code
1064-1963(199910)21:7<1203:ARMBEH>2.0.ZU;2-W
Abstract
The aim of this study was to evaluate arterial relaxation mediated by endot helium-derived hyperpolarizing factor (EDHF) during chronic inhibition of n itric oxide (NO) synthase. We measured the isometric tension of isolated me senteric arteries of Wistar rats administered N-omega-nitro-L-arginine meth yl ester (L-NAME, 100 mg/Kg/day) for 3 weeks. Relaxation to acetylcholine ( ACh) was reduced in L-NAME treated rats (maximum relaxation, 52% versus 79% ). After acute superfuison of 1x10(-4) M L-NAME, half the relaxation was in hibited in controls, while the relaxation was not changed in L-NAME treated rats. In contrast, relaxation to nitroprusside was normal in L-NAME treate d rats. Superfusion of 1x10(-6) M apamin, which inhibits the effects of EDH F, reduced the relaxation. The relaxation inhibited by apamin was not signi ficantly different between the two groups. These findings suggested that in endothelial cells, the synthesis of EDHF is unchanged during a chronic def iciency of relaxation influence of NO.