P. Casaccia-bonnefil et al., Loss of p27Kip1 function results in increased proliferative capacity of oligodendrocyte progenitors but unaltered timing of differentiation, DEVELOPMENT, 126(18), 1999, pp. 4027-4037
In many tissues, progenitor cells permanently withdraw from the cell cycle
prior to commitment towards a differentiated phenotype, In the oligodendroc
yte lineage a counting mechanism has been proposed, linking the number of c
ell divisions to growth arrest and differentiation. A direct prediction of
this model is that an increase in the number of cell divisions would result
in a delayed onset of differentiation. Since the cell cycle inhibitor p27K
ip1 is an essential component of the machinery leading to oligodendrocyte p
rogenitor growth arrest, we examined the temporal relationship between cell
cycle withdrawal and expression of late differentiation markers in vivo, i
n mice carrying a targeted deletion in the p27Kip1 gene. Using bromodeoxyur
idine to label proliferating cells, quaking (QKI) to identify embryonic gli
al progenitors, NG2 to identify neonatal oligodendrocyte progenitors, and m
yelin basic protein to label differentiated oligodendrocytes, we found an i
ncreased number of proliferating QKI- and NG2-positive cells in germinal zo
nes of p27Kip1(-/-) mice at the peak of gliogenesis, However, no delay was
observed in these mice in the appearance of the late differentiation marker
myelin basic protein in the developing corpus callosum and cerebellum. Sig
nificantly, a decrease in cyclin E levels was observed in the brain of p27K
ip1 null mice coincident with oligodendrocyte growth arrest. We conclude th
at two distinct modalities of growth arrest occur in the oligodendrocyte li
neage: a p27Kip1-dependent mechanism of growth arrest affecting proliferati
on in early phases of gliogenesis, and a p27Kip1-independent event leading
to withdrawal from the cell cycle and differentiation.