We examined whether the playback of a chorus song style (i.e., two son
gs presented simultaneously from a single speaker) influences sexual s
ong preferences of female domesticated canaries, Serinus canaria. Six
females were treated with estradiol and their responses to the differe
nt types of song were measured as the frequency of copulation solicita
tion displays. Females responded preferentially to a chorus song style
and reacted less to presentation of single songs (conspecific and het
erospecific). These observations suggest that females' sexual response
s are elicited by two songs heard simultaneously. Our results are disc
ussed in relation to current views on the song preferences of females
and the phenomenon of chorus singing in songbirds.