Immunolocalization of bone morphogenetic protein-2 and-3 and osteogenic protein-1 during murine tooth root morphogenesis and in other craniofacial structures
G. Thomadakis et al., Immunolocalization of bone morphogenetic protein-2 and-3 and osteogenic protein-1 during murine tooth root morphogenesis and in other craniofacial structures, EUR J OR SC, 107(5), 1999, pp. 368-377
The distribution of Bone Morphogenetic Protein-2, and -3 (BMP-2 and BMP-3)
and Osteogenic Protein-1 (OP-1, also known as BMP-7) during root morphogene
sis and in other craniofacial structures was examined in sections of 12- to
18-d-old mouse heads using polyclonal and monoclonal antibodies. BMP-3 and
OP-1 were localized in alveolar bone, cementum, and periodontal ligament,
whereas BMP-2 was only localized in the alveolar bone of periodontium. All
three BMPs were localized in predentine, dentine, odontoblasts, osteoblasts
, osteocytes, osteoid, cartilage, chondrocytes and spiral limbus. BMP-2 and
OP-1 were also localized in spiral ligament and interdentate cells of the
cochlea, whilst BMP-S was restricted to the spiral ganglion. BMP3 was also
localized in ducts of submandibular and sublingual salivary glands, acini o
f the lacrimal gland, Purkinje cells in the cerebellum, nerve fibres of the
cerebellum and brain, afferent cells of the dorsal root ganglia, inferior
alveolar nerve, and peripheral processes of the vestibulocochlear nerve. OP
-1 was also localized in hair and whisker follicles, sclera of the eye and
in ameloblasts. The demonstration of BMP-S in the nervous system suggests t
hat this protein may be neurotrophic during development and maintenance of
the nervous system. The composite expression of BMPs/OPs during periodontal
tissue morphogenesis suggests that optimal therapeutic regeneration may en
tail the combined use of different BMPs/OPs.