Voltage-dependent K+ channels were identified and characterized in primary
culture of rat prostate epithelial cells, A voltage-dependent, inactivating
K+ channel was the most commonly observed ion channel in both lateral and
dorsal cells, The K+ current exhibited a voltage threshold at -40 mV, Avera
ged half-inactivation potential (V-1/2) and the slope factor (k) values wer
e -26 mV and 6, respectively. It showed a monoexponential decay with an ina
ctivation time constant of about 600 ms at +60 mV, The deactivation time co
nstant at -60 mV,vas 30 ms and the reversal potential was estimated at -80
mV, suggesting that current was carried by potassium ions. The scorpion ven
om peptides charybdotoxin (5 nM) and margatoxin (1 nM), inhibited K+ curren
t at all membrane potentials with a rapid and a slow reversibility respecti
vely, Both tetraethylammonium (10 mM) and il-aminopyridine (50 mu M) reduce
d K+ current by similar to 40%, We conclude that plasma membranes of latera
l and dorsal rat prostate epithelial cells contain Ky K+ channels that hare
biophysical and pharmacological properties consistent with those of the Kv
1.3 family. (C) 1999 Federation of European Bioehemical Societies.