Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: Sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE)

In the near future the number of SNPs identified and mapped will increase a nd the need for high throughput SNP typing will be paramount for comprehens ive examination by association of the role of genomic regions in disease tr aits. A range of higher throughput methods for typing SNPs is now in routin e use in many laboratories worldwide, In this report, we analyse the relati ve advantages and disadvantages of three such methods, TaqMan, PCR-SSOP, an d ARMS-MADGE, currently in use in our laboratories. Throughputs achievable are similar, but there are major differences in cost and time for set-up, e quipment, consumables, and staff time, which may determine the choice for i ndividual laboratories. Hum Mutat 14:340-347, 1999. (C) 1999 Wiley Liss, In c.