The modality of enterobacterial common antigen polysaccharide chain lengths is regulated by o349 of the wec gene cluster of Escherichia coli K-12

Citation
K. Barr et al., The modality of enterobacterial common antigen polysaccharide chain lengths is regulated by o349 of the wec gene cluster of Escherichia coli K-12, J BACT, 181(20), 1999, pp. 6564-6568
Citations number
37
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
20
Year of publication
1999
Pages
6564 - 6568
Database
ISI
SICI code
0021-9193(199910)181:20<6564:TMOECA>2.0.ZU;2-Y
Abstract
The assembly of the phosphoglyceride-linked form of enterobacterial common antigen (ECA(PG)) occurs by a mechanism that involves modulation of polysac charide chain length. However, the genetic determinant of this modulation h as not been identified. Site-directed mutagenesis of o349 of the Escherichi a coil K-12 wec gene cluster revealed that this locus encodes a Wzz protein that specifically modulates the chain length of EGA(PG) polysaccharides, a nd we have designated this locus wzz(ECA). The Wzz(ECA)-mediated modulation of ECA(PG) polysaccharide chains is the first demonstrated example of Wzz regulation involving a polysaccharide that is not linked to the core-lipid A structure of lipopolysaccharide.