A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody

Aim-To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum. Methods-latex bead coating tvas adjusted to give maximum sensitivity by att ending to latex size, MPO to latex ratio for coupling, ratio of diluted ser um to MPO-latex, reaction time and temperature for coupling, and reaction t ime for agglutination. Inhibition studies were performed using IMPO, protei nase 3, bactericidal/permeability increasing protein, and lactoferrin. Results-There was very good correlation between this test and the conventio nal anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positiv e in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated bette r with IgM anti-MPO than with IgG anti-MPO. Conclusions-MPO-LSAT is a simple diagnostic rest that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseas es.