Absence of epithelial immunoglobulin A transport, with increased mucosal leakiness, in polymeric immunoglobulin receptor/secretory component-deficient mice
Fe. Johansen et al., Absence of epithelial immunoglobulin A transport, with increased mucosal leakiness, in polymeric immunoglobulin receptor/secretory component-deficient mice, J EXP MED, 190(7), 1999, pp. 915-921
Mucosal surfaces are protected specifically by secretory immunoglobulin A (
SIgA) and SIgM generated through external translocation of locally produced
dimeric IgA and pentameric IgM. Their active transport is mediated by the
epithelial polymeric Ig receptor (pIgR), also called the transmembrane secr
etory component. Paracellular passive external transfer of systemic and loc
ally produced antibodies also provides mucosal protection, making the biolo
gical importance of secretory immunity difficult to assess. Here we report
complete lack of active external IgA and IgM translocation in pIgR knockout
mice, indicating no redundancy in epithelial transport mechanisms. The kno
ckout mice were of normal size and fertility but had increased serum IgG le
vels, including antibodies to Escherichia coli, suggesting undue triggering
of systemic immunity. Deterioration of their epithelial barrier function i
n the absence of SIgA (and SIgM) was further attested to by elevated levels
of albumin in their saliva and feces, reflecting leakage of serum proteins
. Thus, SIgA did not appear to be essential for health under the antigen ex
posure conditions of these experimental animals. Nevertheless, our results
showed that SIgA contributes to maintenance of mucosal homeostasis. Product
ion of SIgA might therefore be a variable in the initiation of human immuno
pathology such as inflammatory bowel disease or gluten-sensitive enteropath
y.