Adaptation to promiscuous usage of chemokine receptors is not a prerequisite for human immunodeficiency virus type 1 disease progression

Citation
Amd. Husman et al., Adaptation to promiscuous usage of chemokine receptors is not a prerequisite for human immunodeficiency virus type 1 disease progression, J INFEC DIS, 180(4), 1999, pp. 1106-1115
Citations number
50
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF INFECTIOUS DISEASES
ISSN journal
00221899 → ACNP
Volume
180
Issue
4
Year of publication
1999
Pages
1106 - 1115
Database
ISI
SICI code
0022-1899(199910)180:4<1106:ATPUOC>2.0.ZU;2-6
Abstract
Fifty percent of individuals infected with human immunodeficiency virus typ e 1 (HIV-1) progress to AIDS in the presence of only non-syncytium-inducing (NSI) variants. These rapidly replicating NSI isolates are associated with a high viral load. The question of whether disease progression in the abse nce of syncytium-inducing (SI) HIV-1 variants is associated with an expansi on of the coreceptor repertoire of NSI HIV-1 variants was studied. Biologic al HIV-1 clones were isolated both early and late in infection from progres sors and long-term survivors with wild-type or mutant CCR5 or CCR2b genotyp es and analyzed for their capacity to use CCR1, CCR2b, CCR3, CCR5, and CXCR 4 on U87 cells coexpressing CD4, All HIV- clones were restricted to the use of CCR5. Absent replication of all HIV-I clones in peripheral blood mononu clear cells from a CCR5 Delta 32 homozygous blood donor confirmed this resu lt. These findings indicate that an expanded coreceptor repertoire of HIV-1 is not a prerequisite for a progressive clinical course of HIV-1 infection .