Suppression of diagonal peaks in TROSY-type H-1 NMR NOESY spectra of N-15-labeled proteins

A novel method for suppression of diagonal peaks in the amide region of NOE SY NMR spectra of N-15-labeled proteins is presented. The method is particu larly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation mechanisms result s in large TROSY effects, i.e., large differences in H-1(N) Linewidths depe nding on the spin state of attached N-15 nuclei. In this limit the new TROS Y NOESY method does not compromise sensitivity, It is demonstrated using a perdeuterated N-15-labeled protein sample, Neural Cell Adhesion Molecule 21 3-308 (NCAM) from rat, in H2O at 800 MHz. (C) 1999 Academic Press.