Quantitative measurement of long range nuclear Overhauser enhancement

Measurement of nuclear Overhauser enhancement is one of the mainstays of st ructural studies of molecules in solution. Measurements, particularly over long distances, are often compromised by spin diffusion. A robust and accur ate method for measuring nuclear Overhauser enhancements that are largely f ree of spin diffusion effects is presented. The effects of using imperfect radiofrequency pulses are considered and experimental data are presented fo r Amphotericin B, (C) 1999 Academic Press.