Enzymatic immobilisation in kappa-carrageenan gel suitable for organic phase enzyme electrode (OPEE) assembly

Organic phase enzyme electrodes (OPEEs) are a new class of biosensors that have extended the field of application of biosensors. The use of biosensors in organic solvent actually makes it possible to analyse insoluble or scar cely soluble substrates in aqueous medium. The best approach for obtaining a new OPEE starts from the optimisation of the experimental conditions in o rder to obtain the maximum activity and stability of the enzyme to be coupl ed to the transducer. In a previous paper we therefore optimised the choice of the solvent, while in this paper a new enzyme immobilisation method in kappa-carrageenan gel and its application to the construction of four diffe rent mono- or bienzymatic OPEEs are described and evaluated. (C) 1999 Elsev ier Science B.V. All rights reserved.