Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells

The purpose of this study was to evaluate the toxicological effects of 4-hy droxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells usi ng the model morphogenetic system of Daunts carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferativ e growth and embryogenesis in D, carota cultures. However, embryogenic capa city was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to t hose which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures, were observed in relation to the d elivery of aldehydes in different culture media. This paper reports, for th e first time, the toxicological effects of HNE and MDA on in vitro plant ce lls. The significance of these findings in relation to the control of in vi tro development in plant tissue cultures which are used in biotechnology pr ogrammes is discussed.