Peptide extract from Brie cheese was examined. The opioid peptide was isola
ted from the extract. Its amino acid composition and N- and C-terminal amin
o acids were determined. Eased on these analyses, the isolated peptide was
assumed to correspond to beta-casomorphin-7 (Tyr-Pro-Phe-Pro-Gly-Pro-IIe).
The opioid activity was measured by examining the effects of peptide extrac
t and isolated opioid peptide on the motor activity of isolated rabbit inte
stine. (C) 1999 Society of Chemical Industry.