DNA condensation and transfection of cells in culture by a new polynorbornane polycationic polymer

Authors
Serres, E Vicendo, P Perez, E Noel, T Rico-Lattes, I
Citation
E. Serres et al., DNA condensation and transfection of cells in culture by a new polynorbornane polycationic polymer, LANGMUIR, 15(20), 1999, pp. 6956-6960
Citations number
22
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
LANGMUIR
ISSN journal
07437463 → ACNP
Volume
15
Issue
20
Year of publication
1999
Pages
6956 - 6960
Database
ISI
SICI code
0743-7463(19990928)15:20<6956:DCATOC>2.0.ZU;2-9
Abstract
We describe the synthesis of a new type of linear polycationic polynorborna ne by vinylic polymerization in water of an ammonium norbornene as monomer. This polymerization catalyzed by PdCl2 leads to stretched flexible polymer s able to complex DNA. Moreover the complexes obtained are stable, protecti ng DNA from DNAse enzymatic degradation. Finally the first transfection ass ays showed that this new polymer is able to mediate transfection in target COS cells.