L. Xerri et al., Quantitative analysis detects ubiquitous expression of apoptotic regulators in B cell non-Hodgkin's lymphomas, LEUKEMIA, 13(10), 1999, pp. 1548-1553
To determine whether the expression levels of Bcl-2 family apoptotic regula
tors are correlated with the histopathological heterogeneity of B cell non-
Hodgkin's lymphomas (NHL), we quantified their expression in malignant B ce
ll populations isolated from 33 biopsy samples, including small lymphocytic
lymphoma (SLL, n = 9), mantle cell lymphoma (MCL, n = 8), follicular lymph
oma (FL, n = 8), and diffuse large cell lymphoma (DLCL, n = 8). Normal B ce
lls purified from reactive lymph nodes and tonsil (n = 3) were used as cont
rols. Cell lysates were analyzed by Western blotting, and signals quantifie
d by densitometry. Expression of Bcl-2 and its homologues, Bcl-xL, Bcl-xS,
Bar, Bad, Bak and Bag-1, was detected in all NHL cases, with wide variation
s between histological subtypes and within each subtype. Statistically sign
ificant differences were: (1) a higher level of Bad expression in DLCL comp
ared to FL and MCL; (2) a lower level of Bak expression in FL compared to D
LCL, SLL and MCL; and (3) a higher Bag-1 expression level in FL compared to
SLL. When compared to NHL cells, normal B cells showed a higher level of B
ar expression, and a lower level of Bcl-xL expression. Thus, quantitative a
nalysis shows ubiquitous expression of Bcl-2 family proteins in normal and
neoplastic B cells; the variations in expression levels may contribute to b
oth the B-NHL clinicopathological diversity and the different apoptotic sen
sitivities of normal B cells vs B-NHL cells.