Sd. Wilson et al., Assessment of the functional integrity of the humoral immune response: Theplaque-forming cell assay and the enzyme-linked immunosorbent assay, METHODS, 19(1), 1999, pp. 3-7
The plaque-forming cell (PFC) assay and enzyme-linked immunosorbent assay (
ELISA) appear to have comparable sensitivity and reproducibility for measur
ing IgM antibody production in mice and rats immunized with sheep red blood
cells (sRBCs). Both assays can be manipulated, with respect to the immuniz
ing antigen (e.g., T-dependent vs T-independent antigen), to provide eviden
ce as to which cell type(s) may be adversely affected by a given compound.
However, the pro assay has more utility in dissecting out the target cell(s
) involved. Since both the PFC assay and the ELISA may be readily conducted
in the rat, it is feasible to incorporate either of these assays into stan
dard acute and repeat dose toxicology studies. This may be accomplished by
inclusion of satellite groups in the study. However, it has been suggested
that the primary antibody response to sRBCs, as measured by an ELISA, may a
lso be evaluated in the main group of animals in a toxicology study without
compromise to the integrity of other toxicological endpoints (e.g., hemato
logy, clinical chemistry, histopathology). Both approaches will provide a m
ore extensive delineation of the safety profile of a drug or chemical. The
latter approach will also reduce the number of animals needed and the cost
of the study.