cDNA isolation, expression, and chromosomal localization of the mouse Pcphproto-oncogene

We report here the isolation and characterization of a cDNA from mouse thym us encoding the murine homolog of the protein product of the Syrian hamster Pcph proto-oncogene. The single open reading frame identified in the cDNA sequence encoded a protein predicted to have 428 amino acids, which shared 93.7% amino acid identity with the Syrian hamster Pcph within the first 412 residues but had a shorter, highly dissimilar C-terminus. Northern and wes tern analyses revealed that Pcph mRNA and protein were widely distributed i n mouse embryo and adult tissues, with the highest expression in adults det ected in kidney and liver. The mouse Pcph proto-oncogene was mapped by link age analysis to within 3.3 +/- 2.3 cM of Pkch-rs1 I on chromosome 12. These data should prove valuable in designing studies to define the cellular fun ction of the Pcph proto-oncogene. (C) 1999 Wiley-Liss, Inc.