Sustained potentiation of AP1 DNA binding is not always associated with neuronal death following systemic administration of kainic acid in murine hippocampus

Citation
T. Kitayama et al., Sustained potentiation of AP1 DNA binding is not always associated with neuronal death following systemic administration of kainic acid in murine hippocampus, NEUROCHEM I, 35(6), 1999, pp. 453-462
Citations number
35
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROCHEMISTRY INTERNATIONAL
ISSN journal
01970186 → ACNP
Volume
35
Issue
6
Year of publication
1999
Pages
453 - 462
Database
ISI
SICI code
0197-0186(199912)35:6<453:SPOADB>2.0.ZU;2-I
Abstract
Mice were intraperitoneally injected with kainic acid (KA), followed by dis section of frozen coronal sections and subsequent punching out of the pyram idal and granular cell layers in the hippocampus under a binocular microsco pe. Systemic administration of KA resulted in marked and sustained potentia tion of binding of a radiolabeled double stranded oligonucleotide probe for the nuclear transcription factor activator protein-1 (AP1) in the pyramida l cell layers of the CA1 and CA3 subfields and the granule cell layers of t he dentate gyrus 2-18 h later. Morphological evaluation using cresyl violet revealed marked losses of neuronal layers in the pyramidal CA1 and CA3 sub fields, but not in the granular dentate gyrus, within 6 weeks after adminis tration. Supershift analysis using antibodies against different Jun and Fos family members differentiated between AP1 DNA binding in hippocampal nucle ar extracts obtained 2 and 18 h after the administration of KA. These resul ts suggest that neuronal death may not always follow modulation of de novo synthesis of particular proteins through sustained potentiation of AP1 DNA binding which involves expression of different Jun and Fos family members i n response to systemic administration of KA in murine hippocampus. (C) 1999 Elsevier Science Ltd. All rights reserved.