CONSTRUCTION OF A YEAST-STRAIN DELETED FOR THE TRP1 PROMOTER AND CODING REGION THAT ENHANCES THE EFFICIENCY OF THE POLYMERASE CHAIN REACTION-DISRUPTION METHOD

The sequence of the genome of Saccharomyces cerevisiae was recently de termined. As well as all the informations concerning the structure of the chromosomes the scientific community had to deal with the discover y of dozens of new open reading frames (ORFs) of unknown function. The study of these ORFs requires the development of simple procedures tha t can be used on a large scale. In the framework of a European Pilot P roject we have described a new approach for deleting ORFs. This method is based on transformation with a polymerase chain reaction product b ut is limited by the use of a strain deleted for the auxotropic marker . We present here the construction of a new recipient strain that lack s the TRP1 region and that allows a high efficiency of gene deletion. ((C) 1997 by John Wiley & Sons, Ltd.)