DNA-SEQUENCE ANALYSIS OF A 23-002-BP DNA FRAGMENT OF THE RIGHT ARM OFSACCHAROMYCES-CEREVISIAE CHROMOSOME-VII

We report the sequence of a 23 002 bp fragment located on the right ar m of Saccharomyces cerevisiae chromosome VII. Analysis of this region revealed 14 complete open reading frames (ORFs) with more than 300 bas e pairs. Six of them correspond to previously known genes. G7164 is th e QCR9 gene coding for subunit 9 of the cytochrome c reductase; G7168 is UBR1, encoding an ubiquitin protein ligase; G7522 is the TYS1 gene, which encodes for the tyrosyl tRNA synthetase; G7526 is TFG1, the gen e coding for the RNA polymerase transcription initiation factor TFIIF (factor G); G7538 is the gene HGH1 which encodes a protein related to the mammalian HMG1 and HMG2 proteins. G7542 is the BUB1 gene which enc odes a ser/thr protein kinase involved in spindle assembly during the cell cycle. One of the ORFs, G7553, shares significant homologies with the gene UTR2 from S. cerevisiae. None of the seven remaining ORFs sh ows similarity to any of the sequences within the public databases. Th ree ORFs are internal ORFs of the above-described known genes, and two small ORFs are completely contained in larger ORFs on the complementa ry strand, and therefore probably do not correspond to real genes. Thi s region also contains three genes specifying tRNAs for Leu, Lys and T rp, and several LTR elements. The sequence described in this paper has been deposited in the EMBL data library under the Accession Number X9 9074. ((C) 1997 by John Wiley & Sons, Ltd.)