SEQUENCE-ANALYSIS OF A 10.5 KB DNA FRAGMENT FROM THE YEAST CHROMOSOME-VII REVEALS THE PRESENCE OF 3 NEW OPEN READING FRAMES AND OF A TRNA(THR) GENE

We report the sequence analysis of a 10 531 bp DNA fragment of Sacchar omyces cerevisiae chromosome VII. This sequence contains five complete open reading frames (ORFs)potentially encoding proteins longer than 1 00 amino acids and an incomplete ORF coding for the 3' part of the GCN 5 gene (Georgakopoulos and Thireos, 1992), ORFs G9160 and G9155 corres pond to the genes ENO1 (Holland et al., 1981) and PUP2 (Gergatsou ei a l., 1992) respectively. ORF G9165 codes for a protein which shares sig nificant homology with known proteins present in databases (see below) . The translated sequence of ORF G9170 shows 88% identity to the 6-pho sphogluconate dehydrogenase encoded by the gene 6PGD from S. cerevisia e present in the SwissProt data library (P38720), This indicates that G9170 might code for a second 6-phosphogluconate dehydrogenase. ORF G9 175 codes for a putative new member of the mitochondrial carrier famil y. A hypothetical tRNA(Thr) (TGT) is also present in position 6842-691 3. The complete sequence has been entered in the EMBL data library und er Accession Number U00028. ((C) 1997 by John Wiley & Sons, Ltd.)