Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces

Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier o f phosphatidylcholine (PC) without known function. Proposed functions inclu de the supply of PC required for secretion into bile or lung air space (sur factant) and the facilitation of enzymatic reactions involving PC synthesis or breakdown. To test these functions, we generated knock-out mice unable to make Pc-tp, Remarkably these mice are normal and have no defect in any o f the postulated Pc-tp functions analyzed. The lipid content and compositio n of the bile, as well as lung surfactant secretion and composition, of Pc- tp (-/-) mice, is normal, The lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is abundant in the liver of mouse pups, P c-tp levels decrease > 10-fold around 2 wk after birth, when bile formation starts. In adult mice, Pc-tp levels are high only in epididymis, testis, k idney, and bone marrow-derived mast cells. Absence of Pc-tp in bone marrow- derived mast cells does not affect their lipid composition or PC synthesis and degradation. We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp.