SOURCES OF VARIABILITY IN MUTANT FREQUENCY DETERMINATIONS IN DIFFERENT ORGANS OF LACZ PLASMID-BASED TRANSGENIC MICE - EXPERIMENTAL FEATURESAND STATISTICAL-ANALYSIS
Meti. Boerrigter et J. Vijg, SOURCES OF VARIABILITY IN MUTANT FREQUENCY DETERMINATIONS IN DIFFERENT ORGANS OF LACZ PLASMID-BASED TRANSGENIC MICE - EXPERIMENTAL FEATURESAND STATISTICAL-ANALYSIS, Environmental and molecular mutagenesis, 29(3), 1997, pp. 221-229
Sources of variability that may affect the measurement of mutant frequ
encies in lacZ plasmid-based transgenic mice, including rescue-to-resc
ue, animal-to-animal (within organ), and organ-to-organ, were studied
in the context of some of the experimental features that distinguish t
his model From other currently used systems. Statistical analysis of r
epealed determinations of DNA from kidney, spleen, liver, lung, and br
ain indicated that 350,000 colony-forming units from each of three ani
mals should be analyzed per treatment group in order to detect a 50% d
ifference in mutant frequencies. Consideration is given to some of the
experimental features of the transgenic assay system, including its p
ositive selection system, its rescue efficiency, the ability to detect
large deletions in the lacZ target genes, the contribution of Escheri
chia coil-derived mutations to the spontaneous mutant frequencies obse
rved in vivo, and cost effectiveness of mutant frequency determination
s. (C) 1997 Wiley-Liss, Inc.