Two anther-expressed genes downstream of SLG(9): identification of a novelS-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn. rapa) L.

Citation
M. Watanabe et al., Two anther-expressed genes downstream of SLG(9): identification of a novelS-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn. rapa) L., SEX PLANT R, 12(3), 1999, pp. 127-134
Citations number
40
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
SEXUAL PLANT REPRODUCTION
ISSN journal
09340882 → ACNP
Volume
12
Issue
3
Year of publication
1999
Pages
127 - 134
Database
ISI
SICI code
0934-0882(199909)12:3<127:TAGDOS>2.0.ZU;2-O
Abstract
The sporophytic self-incompatibility (SI) system in Brassica campestris (sy n. rapa) is controlled by multiple alleles of a single locus, S, in which S LG and SRK are located. SLG and/or SRK appear to comprise the female compon ent of the recognition reaction of SI. The gene encoding the male component must also be located at the S locus but has not been identified to date. R NA gel blot analysis with regions flanking SLG(9) and SRK9 revealed two ant her-expressed genes 3' of the SLG(9) gene. Two cDNA clones were isolated us ing the 3'-flanking region of SLG(9) as probe. Because of sequence similari ty with the Brassica SLL2 gene, one cDNA clone was designated SLL2-S-9 (for S Locus-Linked 2-S-9 gene). The 5'-region of SLL2-S-9 was not present in o ther SLL2 genes examined. The second gene was novel and was designated SAE1 -S-9 (for S locus Anther Expressed 1-S-9 gene). SAE1-S-9 and SLL2-S-9 were located approximately 2.5 kb and 5.5 kb, respectively, downstream of SLG(9) . A 1.4-kb transcript for SLL2-S9 was detected in both vegetative and repro ductive tissues, whereas 1.9-kb and 2.4-kb transcripts were specifically de tected in the anther of S-9 haplotype. SAE1-S-9 was specifically expressed in the anther at the uninucleate stage. This gene encoded a novel polypepti de of 257 amino acids. Database analysis revealed a homologous sequence in the upstream region of SRK3 of B. oleracea. We discuss possible relationshi ps between SLL2-S-9 and SAE1-S-9 and SI.