Nanogram scale absolute configurational assignment of ceramides by circular dichroism

Authors
Jiang, H Huang, XF Nakanishi, K Berova, N
Citation
H. Jiang et al., Nanogram scale absolute configurational assignment of ceramides by circular dichroism, TETRAHEDR L, 40(43), 1999, pp. 7645-7649
Citations number
31
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
40
Issue
43
Year of publication
1999
Pages
7645 - 7649
Database
ISI
SICI code
0040-4039(19991022)40:43<7645:NSACAO>2.0.ZU;2-M
Abstract
Ceramides play a central role in cell regulation and a variety of signal tr ansduction pathways. A simple sensitive microscale circular dichroic (CD) m ethod utilizing zinc porphyrins has been developed for assigning the absolu te configurations of ceramides at the nanogram scale. The assignment is bas ed on the CD spectra of the bis (zinc porphyrin) derivatives of ceramides w ith and without the presence of 1,3-diaminopropane. This method can also be applied to various sphingolipids after enzymatic cleavage to ceramides. (C ) 1999 Elsevier Science Ltd. All rights reserved.