Synthesis of immunoreagents for measurement of galactosylhydroxylysine

(2S,5R)-(+)-Hydroxylysine (6) was transformed into (-)-succinimidyl ester ( 13) and conjugated to BSA or KLH to form immunogens (2 and 3) for generatio n of anti-galactosylhydroxylysine antibodies. Additionally, treatment of (- )-13 with 6-Fln-CH2NH2 (16) or acridinium derivative (17) and subsequent hy drolysis gave the fluorescent (4) and chemiluminescent (5) tracers, respect ively. These immunoreagents (3,4 and 5,6) are essential for development of assays for diagnosis of osteoporosis. (C) 1999 Elsevier Science Ltd. All ri ghts reserved.