Localization of QTLs for tolerance to Cercospora beticola on sugar beet linkage groups

We present a new linkage map for sugar beet (Beta vulgaris) which has been developed using a population segregating for genetic factors that confer to lerance to the leaf spot fungus (Cercospora beticola), the causal factor of leaf spot disease in sugar beet). In the F-2 population studied, a subset of 36 RFLP probes, mapping on eight out of the nine linkage groups of sugar beet, provided the anchor markers to assign chromosomes. A total of 224 ma rkers, including RFLPs, AFLPs, SCARs and microsatellites, were mapped. Esti mates of leaf damage in F-2 and test-cross families were repeated at differ ent stages of plant development. Each set of data was analysed as such. An average estimate was also considered. QTLs with highly significant LOD scor es revealed both by the F-2 and test-cross analyses were localized on linka ge groups 2, 6 and 9. Linkage groups 4 and 5 gave a clear indication of the presence of a QTL only when F-2 data were considered. One highly significa nt QTL with a LOD of 16.0 was revealed only by the data obtained under cond itions of artificial inoculation. This QTL maps at position 90 on chromosom e 3.