FOURIER-TRANSFORM DETECTION OF SINGLET OXYGEN AND FLUORESCENCE FROM CELL-MEMBRANE BOUND PORPHYRINS

Authors
BOHM F MARSTON G TRUSCOTT TG WAYNE RP
Citation
F. Bohm et al., FOURIER-TRANSFORM DETECTION OF SINGLET OXYGEN AND FLUORESCENCE FROM CELL-MEMBRANE BOUND PORPHYRINS, Journal of the Chemical Society. Faraday transactions, 90(17), 1994, pp. 2453-2454
Citations number
11
Categorie Soggetti
Chemistry Physical","Physics, Atomic, Molecular & Chemical
Journal title
Journal of the Chemical Society. Faraday transactions → ACNP
ISSN journal
09565000
Volume
90
Issue
17
Year of publication
1994
Pages
2453 - 2454
Database
ISI
SICI code
0956-5000(1994)90:17<2453:FDOSOA>2.0.ZU;2-Q
Abstract
A weak luminescence due to singlet oxygen O2(a1DELTA(g)) and a stronge r fluorescence emission from a porphyrin bound to the surface of livin g cells have been obtained using a Fourier-transform interferometric t echnique. The porphyrin fluorescence shows a marked wavelength shift c ompared with that observed when the cells are not present. The singlet oxygen emission is accompanied by cell membrane destruction as shown by the Trypan Blue exclusion test.