Low secretion of tumor necrosis factor alpha, but no other Th1 or Th2 cytokines, by peripheral blood mononuclear cells correlates with chronicity in reactive arthritis
J. Braun et al., Low secretion of tumor necrosis factor alpha, but no other Th1 or Th2 cytokines, by peripheral blood mononuclear cells correlates with chronicity in reactive arthritis, ARTH RHEUM, 42(10), 1999, pp. 2039-2044
Objective. To determine Th1 and Th2 cytokine production in patients with re
active arthritis (ReA) in relation to disease outcome and in comparison wit
h rheumatoid arthritis (RA).
Methods. Secretion of tumor necrosis factor alpha (TNF alpha), interferon-g
amma, interleukin-10 (IL-10), and IL-4 by peripheral blood mononuclear cell
s (PBMC) from 53 patients with early ReA (disease duration <8 weeks, 64% HL
A-B27 positive) and 30 patients with early, untreated RA (disease duration
<6 months) was determined by enzyme-linked immunosorbent assay (ELISA) afte
r ex vivo stimulation. Intracellular cytokine staining with quantification
of positive T cells by fluorescense-activated cell sorting (FACS) was perfo
rmed in 12 ReA patients and 12 Ra patients. In 27 ReA patients, cytokine se
cretion was measured again after 3 months, Patients were followed up for 1
year, and cytokine patterns were correlated with disease duration.
Results. TNF alpha secreted by whole PBMC and by T cells was significantly
lower, by ELISA and by FAGS, in ReA patients than in RA patients, while no
significant differences were detected for the other cytokines, ReA patients
with a disease duration of greater than or equal to 6 months show,ed signi
ficantly lower TNF alpha secretion than patients,vith a disease duration of
<6 months (mean +/- SD 385 +/- 207 pg/ml versus 684 +/- 277 pg/ml; P = 0.0
03), Furthermore, low TNF alpha secretion after 3 months also correlated si
gnificantly with a more chronic course of disease. HLA-B27 positive patient
s secreted less TNF alpha than did those who were B27 negative (338 +/- 214
pg/ml versus 512 +/- 207 pg/ml; P = 0.05), and patients with a more chroni
c course had a higher frequency of B27 positivity (47% versus 80%; P = 0.01
). Among the 27 HLA-B27 positive patients, TNF alpha secretion in those wit
h a disease duration of greater than or equal to 6 months was lower than th
at in the 7 with a disease duration of <6 months (308 +/- 167 pg/ml versus
562 +/- 308 pg/ml; P = 0.04),
Conclusion. Low TNF alpha secretion and HLA-B27 status correlate with longe
r disease duration in ReA patients, possibly with an additive effect. The d
iminished TNF alpha production might reflect a state of relative immunodefi
ciency contributing to bacterial persistence in ReA.