Protection of MLV vector particles from human complement

Authors
Breun, S Salmons, B Gunzburg, WH Baumann, JG
Citation
S. Breun et al., Protection of MLV vector particles from human complement, BIOC BIOP R, 264(1), 1999, pp. 1-5
Citations number
46
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
264
Issue
1
Year of publication
1999
Pages
1 - 5
Database
ISI
SICI code
0006-291X(19991014)264:1<1:POMVPF>2.0.ZU;2-A
Abstract
Murine cell-derived MLV vector particles usually are highly sensitive to hu man complement-mediated lysis. Expression of the human complement inhibitor CD59 on murine packaging cells resulted in partial protection of these cel ls from lysis caused by human complement proteins. Furthermore, CD59 was in corporated into MLV vector particles released by these packaging cells, lea ding to an improved resistance of the virions against human complement-medi ated inactivation. (C) 1999 Academic Press.