Re-analysis of magic angle spinning nuclear magnetic resonance determination of interlamellar waters in lipid bilayer dispersions

A recent method to obtain the number of water molecules of hydration of mul tilamellar lipid vesicles using magic angle spinning nuclear magnetic reson ance has been re-examined. The previous interpretation divided the water in to bulk and interlamellar water and ignored water in defects (lakes) that a re intrinsic to multilamellar lipid vesicles; the result was inconsistent w ith x-ray results for the lipid DOPC. The new interpretation takes advantag e of the reduction of lake water with increased spinning and it uses osmoti c pressure measurements to determine the loss of interlamellar water. The n ew result for DOPC from magic angle spinning is consistent with x-ray resul ts.