In paper, by applying fluorescence quenching method and energy transfer tec
hnique, the interaction of serum albumin(BSA and HSA) with ketorolac and fe
prazone was investigated. The binding sites and binding constants were obta
ined. According to the thermodynamic parameters, the main sorts of binding
force can be known. The results show that two drugs and protein have strong
interactions. The mechanism of quenching belongs to static quenching and t
he main sort of binding force is static-electricity gravitation.