Chiral separation and determination of the optical purity of thiamphenicol-related chiral compound by capillary zone electrophoresis with cyclodextrins as chiral selectors

Capillary zone electrophoresis has been used for the chiral separation of t wo thiamphenicol-related compounds, D-threo-3-(4-methylsufonylphenyl)serina te (MSS) and D-three-2-amino-1-(4 -methylthiophenyl) 1,3-propanediol (AMP), with cyclodextrins (CD) as chiral additives. Of the six CD tested, alpha-C D, beta-CD, gamma-CD, heptakis-2,6-di-O-methyl-beta-CD (DM-beta-CD), heptak is-2,3,6-tri-O-methyl-beta-CD (TM-beta-CD) and 2-hydroxypropyl-beta-CD (HP- beta-CD), use of KP-beta-CD alone resulted in chiral recognition of the ena ntiomers of MSS and AMP. The effects of HP-beta-CD concentration, buffer co ncentration, and applied voltage on chiral resolution were investigated. Un der optimum conditions the enantiomers of both MSS (2S,3R and 2R,3S) and AM P (2R,3R and 2S,3S) could be separated to baseline simultaneously This meth od was used successfully for determination of 0.88% 2S,3S-AMP present as an optical impurity in a sample of 2R,3R-AMP.