LOCATION OF XYLANOLYTIC ENZYMES IN CHAETOMIUM-GLOBOSUM

Localisation of endo beta-1-4-xylan xylanohydrolase and beta-xylosidas e was studied in Chaetomium globosum when the fungus was grown on 1% ( w/v) birchwood xylan. Although both enzymes are present in the soluble fraction of the cell, xylanase activity was membrane bound whereas be ta-xylosidase activity was associated with the cell wall. Xylanase and beta-xylosidase acted synergistically on the xylan backbone with the appearance of xylo-oligosaccharides, xylobiose and xylose.